Peshkov I
Vet Med Nauki. 1979;16(2):52-9.
The gel precipitation test after Ouchterlony and Mancini was employed to study comparatively the antigenic structures of S and L forms of Brucella abortus 544. Reduction of the antigenic fractions was established with the L-transformed Brucella organisms as compared to the initial cultures. Reduced were mainly fractions of the antigenic structures that took part in cell wall building. Cytoplasmic antigens remained unchanged. Those that participated in the building of cell walls precipitated in compact lines. On the other hand, the cytoplasmic antigens produced diffuse-and-compact precipitation zones. The intact antigenic structures retained their specificity.
采用奥克特洛尼和曼西尼凝胶沉淀试验,对流产布鲁氏菌544的S型和L型抗原结构进行了比较研究。与原始培养物相比,L型转化的布鲁氏菌生物体的抗原成分减少。减少的主要是参与细胞壁构建的抗原结构成分。细胞质抗原保持不变。参与细胞壁构建的抗原成分在致密条带中沉淀。另一方面,细胞质抗原产生弥散且致密的沉淀区。完整的抗原结构保留了它们的特异性。