Corbel M J
Dev Biol Stand. 1976;31:115-22.
Ribosomes were isolated from Brucella abortus strains 19 and 45/20 by disruption of the cells followed by differential ultracentrifugation. The ribosome preparations contained 2-3 components reacting in immunodiffusion tests but were free of detectable lipopolysaccharide-protein agglutinogen. They crossreacted with antisera to Br. abortus, Br. melitensis, Br. suis and Br. ovis and elicited intradermal delayed hypersensitivity reactions in animals infected with Br. abortus, Br. melitensis or Br. suis. The ribosomes were antigenic in rabbits, guinea pigs and mice. Those from Br. abortus S19 induced agglutinins reaction with smooth brucella strains whereas those from Br. abortus 45/20 induced agglutinins reacting with rough brucella strains. Cattle vaccinated with S19 or 45/20 vaccines or infected with Br. abortus developed pricipitins to ribosomal components at an early stage in the immune response.
通过细胞破碎和差速超速离心从流产布鲁氏菌19株和45/20株中分离核糖体。核糖体制剂在免疫扩散试验中有2 - 3种成分发生反应，但未检测到脂多糖 - 蛋白凝集原。它们与流产布鲁氏菌、马尔他布鲁氏菌、猪布鲁氏菌和绵羊布鲁氏菌的抗血清发生交叉反应，并在感染流产布鲁氏菌、马尔他布鲁氏菌或猪布鲁氏菌的动物中引发皮内迟发型超敏反应。这些核糖体在兔、豚鼠和小鼠中具有抗原性。来自流产布鲁氏菌S19的核糖体诱导与光滑布鲁氏菌菌株发生凝集素反应，而来自流产布鲁氏菌45/20的核糖体诱导与粗糙布鲁氏菌菌株发生凝集素反应。用S19或45/20疫苗接种或感染流产布鲁氏菌的牛在免疫反应的早期阶段产生了针对核糖体成分的沉淀素。
