对从各种聚合物和生物基质中释放的碱性成纤维细胞生长因子的生物活性进行体外评估。

In vitro assessment of the biological activity of basic fibroblast growth factor released from various polymers and biomatrices.

作者信息

Davies M J, Mitchell C A, Maley M A, Grounds M D, Harvey A R, Plant G W, Wood D J, Hong Y, Chirila T V

机构信息

Department of Pathology, University of Western Australia, Nedlands.

出版信息

J Biomater Appl. 1997 Jul;12(1):31-56. doi: 10.1177/088532829701200103.

DOI:10.1177/088532829701200103

PMID:9285329

Abstract

The kinetics of controlled release of basic fibroblast growth factor (bFGF) from polymers (sutures, polycarbonate, Hydron, and Elvax), biopolymers (alginate), and biomatrices (lens capsules), and conditions for storage of bFGF (temperature, plastic type, heparin) were evaluated in vitro. Tissue culture proliferation bioassays with 3T3 fibroblasts, showed that only lens capsules with bFGF had a sustained release of bFGF for up to three weeks. The other materials released all of the 'bound' bFGF with two hours or produced an inflammatory response in vivo. Therefore, the lens tissue had the most potential for controlled long-term delivery of bFGF in vivo. These studies emphasise the importance of in vitro analysis of release kinetics of growth factors from a range of materials as a basis for potential in vivo applications.

摘要

在体外评估了碱性成纤维细胞生长因子（bFGF）从聚合物（缝线、聚碳酸酯、Hydron和聚乙烯醋酸乙烯酯）、生物聚合物（藻酸盐）和生物基质（晶状体囊）中的控释动力学，以及bFGF的储存条件（温度、塑料类型、肝素）。用3T3成纤维细胞进行的组织培养增殖生物测定表明，只有含bFGF的晶状体囊能持续释放bFGF长达三周。其他材料在两小时内释放了所有“结合”的bFGF，或在体内产生了炎症反应。因此，晶状体组织在体内长期控释bFGF方面最具潜力。这些研究强调了对一系列材料中生长因子释放动力学进行体外分析作为潜在体内应用基础的重要性。