Nicaeus T E, Tolentino M J, Adamis A P, Rubin P A
Massachusetts Eye & Ear Infirmary, Boston 02114, USA.
Ophthalmic Plast Reconstr Surg. 1996 Dec;12(4):235-9. doi: 10.1097/00002341-199612000-00004.
Enhanced ingrowth of fibrovascular tissue into alloplastic orbital implants is clinically desirable. Basic fibroblast growth factor (bFGF) is an angiogenic factor that promotes proliferation of endothelial cells. Sucralfate is known to bind bFGF and render it stable by protecting it from degradation. To test the ability of bFGF to stimulate endothelial cell proliferation, porous orbital implants coated with a sustained-release and bioactively-stabilized preparation of the angiogenic peptide bFGF were studied. Hydroxyapatite (HA) and porous polyethylene (PP) implant discs (15 x 3 mm) were coated with sustained-release polymer polyhydroxyethylmethacrylate (hydron), sucralfate (a bFGF stabilizer), hydron plus or hydron/sucralfate plus bFGF. Discs were placed in tissue culture wells plated with 50,000 endothelial cells/well. After 5 days, cells were trypsinized and counted electronically using a Coulter counter. Statistical analysis was performed using unpaired Student's t-test. Implant discs coated with hydron/sucralfate/bFGF had significantly increased endothelial cell proliferation compared to discs coated with hydron alone or hydron/sucralfate (p < 0.05). There was no significant difference in the degree of enhanced proliferation between the HA and PP implants treated with hydron/sucralfate/bFGF (p > 0.05). Minimal proliferation occurred around discs treated with hydron alone or hydron/sucralfate. Coating both HA and PP orbital implants with the sustained-release form of sucralfate/bFGF promoted endothelial cell proliferation in vitro. The enhanced proliferation with hydron/sucralfate/bFGF warrants further exploration in an in vivo model.
纤维血管组织向异体眼眶植入物的内生长增强在临床上是可取的。碱性成纤维细胞生长因子（bFGF）是一种促进内皮细胞增殖的血管生成因子。已知硫糖铝能结合bFGF并通过保护其不被降解而使其稳定。为了测试bFGF刺激内皮细胞增殖的能力，研究了涂覆有血管生成肽bFGF的缓释和生物活性稳定制剂的多孔眼眶植入物。羟基磷灰石（HA）和多孔聚乙烯（PP）植入盘（15×3毫米）分别涂覆有缓释聚合物聚甲基丙烯酸羟乙酯（hydron）、硫糖铝（一种bFGF稳定剂）、hydron加或hydron/硫糖铝加bFGF。将圆盘放入每孔接种50000个内皮细胞的组织培养孔中。5天后，用胰蛋白酶消化细胞并使用库尔特计数器进行电子计数。使用未配对的学生t检验进行统计分析。与单独涂覆hydron或hydron/硫糖铝的圆盘相比，涂覆有hydron/硫糖铝/bFGF的植入圆盘内皮细胞增殖显著增加（p<0.05）。用hydron/硫糖铝/bFGF处理的HA和PP植入物之间的增殖增强程度没有显著差异（p>0.05）。单独用hydron或hydron/硫糖铝处理的圆盘周围发生的增殖极少。用硫糖铝/bFGF的缓释形式涂覆HA和PP眼眶植入物均可促进体外内皮细胞增殖。hydron/硫糖铝/bFGF增强的增殖值得在体内模型中进一步探索。
