Weiss J N
Department of Medicine (Cardiology), UCLA School of Medicine, Los Angeles, California 90095-1760, USA.
FASEB J. 1997 Sep;11(11):835-41.
The Hill coefficient is commonly used to estimate the number of ligand molecules that are required to bind to a receptor to produce a functional effect. However, for a receptor with more than one ligand binding site, the Hill equation does not reflect a physically possible reaction scheme; only under the very specific condition of marked positive cooperativity does the Hill coefficient accurately estimate the number of binding sites. The Hill coefficient is best thought of as an "interaction" coefficient, reflecting the extent of cooperativity among multiple ligand binding sites. Several relatively simple, physically plausible reaction schemes are shown here to produce a variety of ligand dose-response curve phenotypes more appropriately suited to modeling ligand-receptor interactions, especially if independent information about the stochiometry of the ligand-receptor interaction is available.
希尔系数通常用于估计与受体结合以产生功能效应所需的配体分子数量。然而，对于具有多个配体结合位点的受体，希尔方程并不能反映实际可能的反应机制；只有在非常特殊的显著正协同性条件下，希尔系数才能准确估计结合位点的数量。最好将希尔系数视为一种“相互作用”系数，反映多个配体结合位点之间的协同程度。这里展示了几种相对简单、符合物理原理的反应机制，以产生更适合模拟配体-受体相互作用的各种配体剂量-反应曲线表型，特别是在有关于配体-受体相互作用化学计量的独立信息时。
