Mapelli M, Greco F, Gussoni M, Consonni R, Zetta L
Istituto di Chimica delle Marcomolecole, CNR, Milano, Italy.
Magn Reson Imaging. 1997;15(6):693-700. doi: 10.1016/s0730-725x(97)00006-4.
Zero (ZQ) and double (DQ) quantum 2D chemical shift selective and spin-echo 3D NMR imaging at microscopy resolution, has been applied to the morphological study of silkworm, Bombyx mori, during its metamorphosis. Attention had been focused on the evolution of the internal structure of the insect during its postembryonal life occurring through the larval, pupal and adult development. A major objective of this work was the characterization of the silk glands, responsible for the synthesis and secretion of fibroin and sericin, through the changes of distribution and mobility of water, by imaging the water protons during postembryonal life stages. Moreover, alanine deriving from silk gland proteins was imaged during the last life stage of Bombyx mori.
具有显微镜分辨率的零量子（ZQ）和双量子（DQ）二维化学位移选择性及自旋回波三维核磁共振成像技术成像成像技术，已被应用于家蚕（Bombyx mori）变态发育过程的形态学研究。研究重点关注昆虫胚胎后发育阶段，即幼虫、蛹和成虫发育过程中内部结构的演变。这项工作的一个主要目标是，通过在胚胎后发育阶段对水质子成像，根据水的分布和流动性变化，来表征负责丝素蛋白和丝胶蛋白合成与分泌的丝腺。此外，还对家蚕最后一个生命阶段丝腺蛋白衍生的丙氨酸进行了成像。
