Millot M C, Sébille B, Mangin C
Laboratoire de Physico-Chimie des Biopolymères, UMR 27, CNRS, Université Paris XII, Thiais, France.
J Chromatogr A. 1997 Jul 25;776(1):37-44. doi: 10.1016/s0021-9673(97)00156-8.
Human serum albumin (HSA) was bound to porous silica, using a reactive polymer derived from polymethacryloyl chloride. Two different procedures were used for coating silica with the polymer. In the first method, the polymer was deposited onto amino-silica by reaction between its reactive functions and NH2 groups on silica. In the second method, the monomer was first linked to the amino-silica and copolymerization with the excess of monomer initiated thereafter. The enantiomeric properties of the resulting supports after the coupling of HSA were compared using different mobile phases. The higher amount of HSA bound using the later method, resulted in higher retention of the enantiomers and better enantioselectivity.
