用于蛋白质纯化的聚苯乙烯基质的改性。II. 戊二醛 - 聚乙烯醇交联度对各种染料配体色谱系统的影响。

Modification of polystyrenic matrices for the purification of proteins. II. Effect of the degree of glutaraldehyde-poly(vinyl alcohol) crosslinking on various dye ligand chromatography systems.

作者信息

Nash D C, Chase H A

机构信息

Department of Chemical Engineering, University of Cambridge, UK.

出版信息

J Chromatogr A. 1997 Jul 25;776(1):55-63. doi: 10.1016/s0021-9673(97)00469-x.

DOI:10.1016/s0021-9673(97)00469-x

PMID:9286078

Abstract

A poly(styrene-divinylbenzene) chromatography matrix, CG1000sd (TosoHaas) has been modified by the adsorption and crosslinking of poly(vinyl alcohol) (PVA) to create a matrix suitable for the attachment of dye ligands for the adsorption of lysozyme. However, it is shown that there was limited recovery and repeated drops in capacity with adsorption of human serum albumin (HSA). The effect of changing the nature of the PVA crosslinking on the HSA binding characteristics was studied, as well as the effect of using differing dye ligands. The total amount of irreversible HSA binding decreased with greater crosslinking and there were large differences in HSA adsorption characteristics between differing dye types.

摘要

一种聚（苯乙烯-二乙烯基苯）色谱基质CG1000sd（托索哈斯公司）已通过聚乙烯醇（PVA）的吸附和交联进行了改性，以创建一种适合连接染料配体用于吸附溶菌酶的基质。然而，研究表明，在吸附人血清白蛋白（HSA）时，回收率有限且容量反复下降。研究了改变PVA交联性质对HSA结合特性的影响，以及使用不同染料配体的影响。随着交联程度的增加，不可逆结合的HSA总量减少，不同染料类型之间的HSA吸附特性存在很大差异。