用于蛋白质纯化的聚苯乙烯基质的改性。III. 聚乙烯醇改性对传统和灌注聚苯乙烯基质上蛋白质吸附特性的影响。

Modification of polystyrenic matrices for the purification of proteins. III. Effects of poly(vinyl alcohol) modification on the characteristics of protein adsorption on conventional and perfusion polystyrenic matrices.

作者信息

Nash D C, Chase H A

机构信息

Department of Chemical Engineering, University of Cambridge, UK.

出版信息

J Chromatogr A. 1997 Jul 25;776(1):65-73. doi: 10.1016/s0021-9673(97)00470-6.

DOI:10.1016/s0021-9673(97)00470-6

PMID:9286079

Abstract

Poly(styrene-divinylbenzene) (PS-DVB) chromatography matrices, CG1000sd 20-50 microns (TosoHaas), PLRP4000s 15-25 microns, PLRP4000s 50-70 microns (Polymer Laboratories) have been modified by the adsorption and crosslinking of poly(vinyl alcohol) (PVA) to create a matrix suitable for the attachment of dye ligands. The adsorption capacities of lysozyme and HSA on these Procion Yellow HE-3G dyed PVA modified PS-DVB matrices were measured at various flow-rates and the capacities were compared with a Procion Yellow HE-3G dyed OH-activated POROS 20, 20-micron matrix (PerSeptive Biosystems). The adsorption of small proteins was not hindered by the smaller pores of the CG1000sd beads, but as protein size increased, and at high flow-rates, a high mass transfer rate became more dependent on large pore size and small particle diameter.

摘要

通过聚乙烯醇（PVA）的吸附和交联对聚（苯乙烯 - 二乙烯基苯）（PS - DVB）色谱基质进行了改性，这些基质包括20 - 50微米的CG1000sd（TosoHaas公司）、15 - 25微米的PLRP4000s以及50 - 70微米的PLRP4000s（Polymer Laboratories公司），以制备适合连接染料配体的基质。在不同流速下测量了溶菌酶和人血清白蛋白（HSA）在这些经普施安黄HE - 3G染色的PVA改性PS - DVB基质上的吸附容量，并将这些容量与经普施安黄HE - 3G染色的20微米的OH活化POROS 20基质（PerSeptive Biosystems公司）进行了比较。CG1000sd微球较小的孔径并未阻碍小蛋白质的吸附，但随着蛋白质尺寸增大以及在高流速下，较高的传质速率变得更依赖于大孔径和小粒径。