Leonard M, Fournier C, Dellacherie E
LCPM, URA CNRS 494, ENSIC, Nancy, France.
J Chromatogr B Biomed Appl. 1995 Feb 3;664(1):39-46. doi: 10.1016/0378-4347(94)00347-8.
A method is described for the hydrophilization of macroporous poly(styrene-divinylbenzene) (PS-DVB) beads by adsorption of polyvinyl alcohol (PVA). PVA adsorbs strongly to PS-DVB surfaces but partially desorbs when exposed to protein solutions. To overcome this problems, the polymeric adsorbed layer was stabilized by crosslinking. We report the effect of polymer adsorption and crosslinking conditions on the amount of adsorbed PVA, on the stability of the polymeric layer and on the hydrophilization efficiency with regard to bovine serum albumin-a strongly hydrophobic protein. The properties of the coated supports were also evaluated by size-exclusion chromatography. PVA coating was shown to greatly reduce hydrophobic interactions. The pore size of modified PS-DVB particles was found to decrease significantly with increasing amounts of adsorbed PVA.
描述了一种通过吸附聚乙烯醇（PVA）使大孔聚（苯乙烯 - 二乙烯基苯）（PS - DVB）珠粒亲水化的方法。PVA强烈吸附在PS - DVB表面，但暴露于蛋白质溶液时会部分解吸。为克服此问题，通过交联使聚合物吸附层稳定。我们报告了聚合物吸附和交联条件对PVA吸附量、聚合物层稳定性以及对牛血清白蛋白（一种强疏水蛋白质）的亲水化效率的影响。还通过尺寸排阻色谱法评估了包覆载体的性能。结果表明，PVA涂层可大大减少疏水相互作用。发现改性PS - DVB颗粒的孔径随着PVA吸附量的增加而显著减小。
