Tan M C, Mommaas A M, Drijfhout J W, Jordens R, Onderwater J J, Verwoerd D, Mulder A A, van der Heiden A N, Ottenhoff T H, Cella M, Tulp A, Neefjes J J, Koning F
Department of Immunohematology, Leiden University Hospital, The Netherlands.
Adv Exp Med Biol. 1997;417:171-4. doi: 10.1007/978-1-4757-9966-8_28.
Dendritic cells (DCs) use macropinocytosis and mannose receptor mediated endocytosis for the uptake of exogenous antigens. Here we show that the endocytosis of the mannose receptor and mannosylated antigen is distinct from that of a non-mannosylated antigen. Shortly after internalization, however, both mannosylated and non-mannosylated antigen are found in an MIIC like compartment. The mannose receptor itself does not reach this compartment, and probably releases its ligand in an earlier endosomal structure. Finally, we found that mannosylation of peptides strongly enhanced their potency to stimulate HLA class II-restricted peptide-specific T cell clones. Our results indicate that mannosylation of antigen leads to selective targeting and subsequent superior presentation by DCs which may be useful for vaccine design.
树突状细胞（DCs）利用巨胞饮作用和甘露糖受体介导的内吞作用摄取外源性抗原。在此我们表明，甘露糖受体和甘露糖基化抗原的内吞作用不同于非甘露糖基化抗原的内吞作用。然而，内化后不久，甘露糖基化和非甘露糖基化抗原都出现在类似MIIC的区室中。甘露糖受体本身不会到达这个区室，可能在早期的内体结构中释放其配体。最后，我们发现肽的甘露糖基化强烈增强了它们刺激HLA II类限制性肽特异性T细胞克隆的能力。我们的结果表明，抗原的甘露糖基化导致DCs的选择性靶向和随后的高效呈递，这可能对疫苗设计有用。
