Engering A J, Cella M, Fluitsma D M, Hoefsmit E C, Lanzavecchia A, Pieters J
Basel Institute for Immunology, Switzerland.
Adv Exp Med Biol. 1997;417:183-7. doi: 10.1007/978-1-4757-9966-8_31.
In an immature state, dendritic cells (DC) can capture antigen via at least two mechanisms. First, DC use macropinocytosis for continuous uptake of large amounts of soluble antigens. Second, they express high levels of mannose receptor that can mediate internalization of glycosylated ligands. We found that dendritic cells can present mannosylated antigen 100-1000 fold more efficiently than non-mannosylated antigen. Immunocytochemistry as well as subcellular fractionation demonstrated that the mannose receptor and MHC class II molecules were located in distinct subcellular compartments. These results demonstrate that the mannose receptor endows DC with a high capacity to present glycosylated antigens at very low concentrations.
在未成熟状态下，树突状细胞（DC）可通过至少两种机制捕获抗原。首先，DC利用巨胞饮作用持续摄取大量可溶性抗原。其次，它们表达高水平的甘露糖受体，该受体可介导糖基化配体的内化。我们发现，树突状细胞呈递甘露糖基化抗原的效率比非甘露糖基化抗原高100 - 1000倍。免疫细胞化学以及亚细胞分级分离表明，甘露糖受体和MHC II类分子位于不同的亚细胞区室。这些结果表明，甘露糖受体赋予DC在极低浓度下呈递糖基化抗原的高能力。
