Drăgan M, Hary M, Dema E
Zahn Mund Kieferheilkd Zentralbl. 1979;67(4):364-8.
Drăgan's high-speed PBT (polychrome blue tannin) staining method enables cellular elements to be detected in oral smears in one minute at most. The basic dyes in the polychrome blue solution allow the acid-group-containing substrate to be detected in the nucleus, in the cytoplasm of epithelial cells and leukocytes, as well as in the elements of the flora. The chromatin shows a distinct basophilic, orthochromatic reaction. The fine, reddish-violet metachromatic granules in the cytoplasm are of a glycolipid nature and occur with the tonofibrils. The keratohyalin granules show a basophilic orthochromatic or metachromatic, homogeneous or granular reaction in relation to the chemically acid portions of their structure.
德拉甘的高速PBT（多色蓝单宁）染色方法能够在最多一分钟内检测出口腔涂片中的细胞成分。多色蓝溶液中的碱性染料可使含酸基团的底物在细胞核、上皮细胞和白细胞的细胞质以及菌群成分中被检测到。染色质呈现出明显的嗜碱性正染色反应。细胞质中细小的红紫色异染颗粒具有糖脂性质，并与张力原纤维一起出现。透明角质颗粒相对于其结构中的化学酸性部分呈现出嗜碱性正染色或异染色、均匀或颗粒状反应。
