[A modified staining method for examining Y chromatin].

A modified staining method for Y chromatin is reported here which proved to be rapid and efficient for sex differentiation diagnosis. Samples were fixed in methanol: acetic acid (3:1) solution and stained with dyes in McIlvaine buffer. Y chromatin in buccal smears from normal males could be clearly revealed by this method. With this modified method and X-chromatin examination, one testicular feminization was found during examination of 1342 athletes for the 11th Asian Games.