Huang E Y, Mohler A M, Rohlman C E
Department of Chemistry, Pomona College, Claremont, California 91711, USA.
J Bacteriol. 1997 Sep;179(17):5648-53. doi: 10.1128/jb.179.17.5648-5653.1997.
Interruption of folate metabolism by trimethoprim results in the elevated expression of folate stress proteins in Escherichia coli. E. coli grown in culture medium supplemented with the folate-dependent metabolites glycine, methionine, and the purine nucleoside inosine shows reduced expression of folate stress proteins. The folate stress proteins include the universal stress protein, the ferric uptake regulatory repressor, and possibly, lipoamide dehydrogenase, the L protein component of the glycine cleavage enzyme complex.
甲氧苄啶对叶酸代谢的干扰会导致大肠杆菌中叶酸应激蛋白的表达升高。在补充了依赖叶酸的代谢物甘氨酸、蛋氨酸和嘌呤核苷肌苷的培养基中生长的大肠杆菌，其叶酸应激蛋白的表达会降低。叶酸应激蛋白包括通用应激蛋白、铁摄取调节阻遏物，可能还包括硫辛酰胺脱氢酶，即甘氨酸裂解酶复合体的L蛋白组分。
