Kim J S, Choi C W, Kim B S, Shin S W, Kim Y H, Mok Y J, Kim J S, Koo B H
Department of Internal Medicine and General Surgery, School of Medicine, Korea University, Seoul, Korea.
J Korean Med Sci. 1997 Aug;12(4):311-5. doi: 10.3346/jkms.1997.12.4.311.
Genetic damages are frequently found in both tumor and normal cells at carcinogen exposed areas in the patients with upper aerodigestive tract cancer. These phenomena are explained by the multistage process and/or field cancerization theories. The c-erbB-2 proto-oncogene has been amplified in many human tumors including breast, stomach, kidney and lung cancers. To study the possible evidence of multistage process and/or field cancerization in the development of gastric adenocarcinoma, the amplification statuses of c-erbB-2 proto-oncogene using the Southern hybridization technique were evaluated at the 45 gastric adenocarcinoma specimen sets consisting of tumor tissue, adjacent normal tissue (within 2 cm of the primary tumor), metastatic tissue and normal stomach tissue (at least 5 cm away from primary tumor). As a result, c-erbB-2 proto-oncogene at 2 specimen sets (4.4%) was amplified 2- to 4-fold to normal control status. In these 2 cases, c-erbB-2 proto-oncogene at histologically normal tissue adjacent to tumor tissue was amplified. And, the metastatic tissue of 1 case also exhibited c-erbB-2 proto-oncogene amplification of which the degree was less than that of tumor tissue. From these results, we were able to suspect that c-erbB-2 proto-oncogene amplification in the normal tissue adjacent to tumor tissue could be a biomarker of premalignant changes in a small proportion of gastric adenocarcinoma patients. And, this result might suggest the possible role of multistage process and/or field cancerization in the development of gastric adenocarcinoma.
在上消化道癌患者中，致癌物暴露区域的肿瘤细胞和正常细胞中经常发现基因损伤。这些现象可以用多阶段过程和/或场癌化理论来解释。c-erbB-2原癌基因在包括乳腺癌、胃癌、肾癌和肺癌在内的许多人类肿瘤中都有扩增。为了研究胃腺癌发生过程中多阶段过程和/或场癌化的可能证据，我们使用Southern杂交技术评估了45组胃腺癌标本中c-erbB-2原癌基因的扩增状态，这些标本包括肿瘤组织、相邻正常组织（距原发肿瘤2厘米以内）、转移组织和正常胃组织（距原发肿瘤至少5厘米）。结果显示，2组标本（4.4%）中的c-erbB-2原癌基因扩增至正常对照状态的2至4倍。在这2例病例中，肿瘤组织相邻的组织学正常组织中的c-erbB-2原癌基因发生了扩增。而且，1例病例的转移组织也显示出c-erbB-2原癌基因扩增，但其程度低于肿瘤组织。从这些结果中，我们怀疑肿瘤组织相邻正常组织中的c-erbB-2原癌基因扩增可能是一小部分胃腺癌患者癌前病变的生物标志物。并且，这一结果可能提示多阶段过程和/或场癌化在胃腺癌发生中的可能作用。
