He Z, Tessier-Lavigne M
Howard Hughes Medical Institute, Department of Anatomy, University of California, San Francisco 94143-0452, USA.
Cell. 1997 Aug 22;90(4):739-51. doi: 10.1016/s0092-8674(00)80534-6.
Extending axons in the developing nervous system are guided to their targets through the coordinate actions of attractive and repulsive guidance cues. The semaphorin family of guidance cues comprises several members that can function as diffusible axonal chemorepellents. To begin to elucidate the mechanisms that mediate the repulsive actions of Collapsin-1/Semaphorin III/D (Sema III), we searched for Sema III-binding proteins in embryonic rat sensory neurons by expression cloning. We report that Sema III binds with high affinity to the transmembrane protein neuropilin, and that antibodies to neuropilin block the ability of Sema III to repel sensory axons and to induce collapse of their growth cones. These results provide evidence that neuropilin is a receptor or a component of a receptor complex that mediates the effects of Sema III on these axons.
在发育中的神经系统中，延伸的轴突通过吸引性和排斥性导向线索的协同作用被引导至其靶标。导向线索的信号素家族包含几个可作为可溶性轴突化学排斥剂发挥作用的成员。为了开始阐明介导Collapsin-1/信号素III/D（Sema III）排斥作用的机制，我们通过表达克隆在胚胎大鼠感觉神经元中寻找Sema III结合蛋白。我们报告Sema III与跨膜蛋白神经纤毛蛋白以高亲和力结合，并且针对神经纤毛蛋白的抗体可阻断Sema III排斥感觉轴突以及诱导其生长锥塌陷的能力。这些结果提供了证据，表明神经纤毛蛋白是介导Sema III对这些轴突作用的受体或受体复合物的一个组分。
