The effect of storage and handling on the activity of lactate dehydrogenase and its isoenzymes in horse serum.

The activity of lactate dehydrogenase and its isoenzymes in normal horse serum was studied under four conditions of storage. For storage periods up to three weeks freezing (-20 degrees C) was most satisfactory whereas for 24 to 48 h room temperature proved optimal. The most unsuitable temperature for lengthy storage was 4 degrees C. Storage of clotted blood for up to 48 h may result in haemolysis and unexpected rise in activity.