Xi K, Burnett P A
Agriculture and Agri-Food Canada, Lacombe Research Center, Alberta, Canada.
Biotech Histochem. 1997 Jul;72(4):173-7. doi: 10.3109/10520299709082234.
Staining of paraffin embedded sections with periodic acid-Schiff reagent and fast green before paraffin removal resulted in differentiation of barley seed and leaf tissue from fungal structures of Rhynchosporium secalis. Crystal violet, toluidine blue O and antiline blue also successfully stained fungal structures of R. secalis in barley leaf tissues. Staining of embedded sections before paraffin removal allows simple processing of a series of sections, saves time and reduces solvent consumption.
在脱石蜡之前，用高碘酸-希夫试剂和固绿对石蜡包埋切片进行染色，可使大麦种子和叶片组织与大麦云纹病菌的真菌结构区分开来。结晶紫、甲苯胺蓝O和苯胺蓝也成功地对大麦叶片组织中的大麦云纹病菌的真菌结构进行了染色。在脱石蜡之前对包埋切片进行染色，便于对一系列切片进行简单处理，节省时间并减少溶剂消耗。
