在进行石蜡提取之前，用俾斯麦棕Y和天青B或甲苯胺蓝O对植物角质层进行染色。

Plant cuticle staining with bismarck brown Y and azure B or toluidine blue O before paraffin extraction.

作者信息

Graham E T, Joshi P A

机构信息

Department of Ornamental Horticulture, The University of Tennessee, Knoxville, 37901-1071, USA.

出版信息

Biotech Histochem. 1996 Mar;71(2):92-5. doi: 10.3109/10520299609117141.

DOI:10.3109/10520299609117141

PMID:9138538

Abstract

Transverse paraffin sections of mature greenwood stems of rose (Rosa x hybrida) and flowering dogwood (Cornus florida L.) were stained with Bismarck brown followed by azure B or toluidine blue 0. The Bismarck brown was replaced by thiazin dye metachromasia in all structures except the cuticle which remained brown or yellow. The interface between the cuticle and exterior cell walls of the epidermis was delineated clearly.

摘要

对玫瑰（Rosa x hybrida）和多花梾木（Cornus florida L.）成熟绿茎的横向石蜡切片先用俾斯麦棕染色，然后用天青B或甲苯胺蓝O染色。除角质层仍为棕色或黄色外，所有结构中的俾斯麦棕均被噻嗪染料异染性取代。角质层与表皮外部细胞壁之间的界面清晰可辨。