Pham K, Smoyer W E, Archer D C, Gabbai F, Kelly C J
Department of Medicine, University of California, San Diego, and the Veterans Affairs Medical Center, 92161, USA.
Kidney Int. 1997 Sep;52(3):725-32. doi: 10.1038/ki.1997.388.
We have examined whether oral feeding of antigen can regulate the expression of autoimmune interstitial nephritis induced by antigen-in-adjuvant (RTA/CFA) immunization of Brown Norway rats. Male rats were divided into six experimental groups: Group I, RTA/CFA immunization alone; Groups II, III, and IV were pretreated with 1 mg (Group II), 5 mg (Group III), and 25 mg (Group IV) of oral tubular antigen every other day for ten days, followed by RTA/CFA immunization; Group V was pretreated with a control antigen, followed by RTA/CFA immunization; and Group VI was immunized with CFA alone. Renal histology, inulin clearance, DTH responses to RTA, and IgG antibody responses to RTA were monitored as endpoints of the study. Our results demonstrated that Group III and IV animals had significantly less severe renal injury, as assessed by inulin clearance and extent of renal cortical involvement by mononuclear cells. Group II and IV animals had suppressed DTH responses, and only Group IV animals had significantly depressed antigen-specific IgG serum titers. Group III animals had neither suppressed DTH responses or IgG titers. We conclude that oral administration of tubular antigen can modulate the intensity of interstitial nephritis produced by immunization, but that the regulatory mechanism is not dependent (at all doses of fed antigen) on suppressed DTH reactivity to RTA or suppressed antigen-specific IgG.
我们研究了经口喂食抗原是否能够调节用抗原佐剂（RTA/CFA）免疫棕色挪威大鼠所诱导的自身免疫性间质性肾炎的表达。雄性大鼠被分为六个实验组：第一组，仅用RTA/CFA免疫；第二、三、四组每隔一天用1毫克（第二组）、5毫克（第三组）和25毫克（第四组）的肾小管抗原预处理十天，随后进行RTA/CFA免疫；第五组先用对照抗原预处理，随后进行RTA/CFA免疫；第六组仅用CFA免疫。监测肾组织学、菊粉清除率、对RTA的迟发型超敏反应（DTH）以及对RTA的IgG抗体反应作为研究的终点。我们的结果表明，通过菊粉清除率和单核细胞累及肾皮质的程度评估，第三组和第四组动物的肾损伤明显较轻。第二组和第四组动物的DTH反应受到抑制，只有第四组动物的抗原特异性IgG血清滴度显著降低。第三组动物的DTH反应和IgG滴度均未受到抑制。我们得出结论，经口给予肾小管抗原能够调节免疫接种所产生的间质性肾炎的强度，但调节机制（在所有喂食抗原剂量下）并不依赖于对RTA的DTH反应性降低或抗原特异性IgG受到抑制。
