Ogura H, Ayata M, Hayashi K, Seto T, Matsuoka O, Hattori H, Tanaka K, Tanaka K, Takano Y, Murata R
Department of Medical Zoology, Osaka City University Medical School, Asahimachi, Japan.
J Neurovirol. 1997 Aug;3(4):304-9. doi: 10.3109/13550289709029472.
Subacute sclerosing panencephalitis virus has been isolated with difficulty from brains of infected patients. More strains are needed for the study of the pathogenesis of this virus. To make the isolation more efficient, we selected portions to be examined from the brains of three patients with reference to findings of repeated magnetic resonance and computed tomographic imaging. Three cell lines susceptible to measles virus field strains were used. In all three cases viruses were isolated most effectively from recent lesions and with Vero cells. Our results suggested that these imaging methods and Vero cells could be used for improvement in the efficiency of isolation of this virus from patient brains.
亚急性硬化性全脑炎病毒很难从受感染患者的大脑中分离出来。研究这种病毒的发病机制需要更多的毒株。为了提高分离效率，我们根据重复磁共振成像和计算机断层扫描成像的结果，从三名患者的大脑中选择了要检查的部位。使用了三种对麻疹病毒野毒株敏感的细胞系。在所有三例中，从近期病变部位并使用非洲绿猴肾细胞（Vero细胞）能最有效地分离出病毒。我们的结果表明，这些成像方法和Vero细胞可用于提高从患者大脑中分离这种病毒的效率。
