[钙浓度对4-氨基吡啶诱导的离体腹膜肥大细胞组胺释放的影响]

[Influence of calcium concentration on 4-aminopyridine induced histamine release from isolated peritoneal mast cells].

作者信息

Xu J H, Wang P, Chen Z G, Zhang J X

机构信息

Department of Pharmacology, Faculty of Pharmaceutical Sciences, Zhejiang Medical University, Hangzhou.

出版信息

Yao Xue Xue Bao. 1995;30(3):235-8.

PMID:7543724

Abstract

4-Aminopyridine (4-AP) has been shown to induce histamine release from isolated peritoneal mast cells (PMC) in mice and rats. In the presence of extracellular calcium at normal level (0.9 mmol.L-1) histamine release induced by 4-AP (13.6 mmol.L-1) from mice PMC was 33.0% +/- 4.6%, while at low calcium concentration (0.5 mmol.L-1) and in calcium-free medium this parameter decreased to 25.5% +/- 4.2% and 16.3% +/- 3.7% respectively. Histamine release in response to 4-AP (10 mmol.L-1) from rat PMC was 39.1% +/- 6.7% (0.9 mmol.L-1 calcium), while at low calcium concentration (0.5 mmol.L-1) and in calcium-free medium this parameter decreased to 29.3% +/- 4.7% and 20.2% +/- 2.9% respectively. Results of statistical analysis indicate that 4-AP induced histamine release is related to Ca2+ concentration. When rat PMC were preincubated in calcium-free medium with EDTA 0.1 mmol.L-1 for 180 min 4-AP induced histamine release was 13.8% +/- 1.6%. This shows that 4-AP also elicited mobilization of endogenous calcium stores in mast cells. The mechanism of 4-AP induced histamine release was discussed.

摘要

4-氨基吡啶（4-AP）已被证明可诱导小鼠和大鼠分离的腹膜肥大细胞（PMC）释放组胺。在细胞外钙处于正常水平（0.9 mmol·L-1）时，4-AP（13.6 mmol·L-1）诱导小鼠PMC释放组胺的量为33.0%±4.6%，而在低钙浓度（0.5 mmol·L-1）和无钙培养基中，该参数分别降至25.5%±4.2%和16.3%±3.7%。大鼠PMC对4-AP（10 mmol·L-1）的组胺释放量为39.1%±6.7%（0.9 mmol·L-1钙），而在低钙浓度（0.5 mmol·L-1）和无钙培养基中，该参数分别降至29.3%±4.7%和20.2%±2.9%。统计分析结果表明，4-AP诱导的组胺释放与Ca2+浓度有关。当大鼠PMC在含0.1 mmol·L-1 EDTA的无钙培养基中预孵育180分钟时，4-AP诱导的组胺释放量为13.8%±1.6%。这表明4-AP还引发了肥大细胞内源性钙库的动员。文中讨论了4-AP诱导组胺释放的机制。