Brattig N, Nietz C, Hounkpatin S, Lucius R, Seeber F, Pichlmeier U, Pogonka T
Department of Clinical Chemistry, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
J Infect Dis. 1997 Sep;176(3):838-42. doi: 10.1086/517317.
Subjects with generalized onchocerciasis (GEN), with the sowdah form, and with exposure but without onchocerciasis (endemic normal/putatively immune; EN/PI) were studied for cytokine responses to Onchocerca volvulus extract (OvAg) and recombinant Ov33 and OvL3-1 proteins. Higher levels of cytokines were produced in response to OvAgs in sowdah and EN/PI than in GEN subjects. Peripheral blood mononuclear cells did not produce interferon-gamma in response to antigens. OvAg induced interleukin (IL)-5, IL-2, granulocyte-macrophage colony-stimulating factor (GM-CSF), and soluble IL-2 receptor. EN/PI and sowdah persons produced significantly more IL-5 and IL-2 than GEN subjects, and EN/PI subjects had significantly higher GM-CSF levels than GEN persons. The low IL-5 and GM-CSF levels in GEN subjects were increased by addition of exogenous IL-2. Ov33 and OvL3-1 stimulated production of IL-10 and less IL-5 and IL-2. The study groups did not show a strict Th2-like cytokine response.
对患有全身性盘尾丝虫病（GEN）、患有sowdah型盘尾丝虫病以及暴露于盘尾丝虫但未患盘尾丝虫病（地方性正常/推定免疫；EN/PI）的受试者，研究了其对盘尾丝虫提取物（OvAg）以及重组Ov33和OvL3 - 1蛋白的细胞因子反应。与GEN受试者相比，sowdah型和EN/PI受试者对OvAg产生的细胞因子水平更高。外周血单核细胞对抗原不产生干扰素 - γ。OvAg可诱导白细胞介素（IL）-5、IL - 2、粒细胞 - 巨噬细胞集落刺激因子（GM - CSF）以及可溶性IL - 2受体的产生。EN/PI和sowdah型患者产生的IL - 5和IL - 2显著多于GEN受试者，且EN/PI受试者的GM - CSF水平显著高于GEN受试者。通过添加外源性IL - 2可提高GEN受试者中较低的IL - 5和GM - CSF水平。Ov33和OvL3 - 1刺激IL - 10的产生，同时减少IL - 5和IL - 2的产生。研究组未表现出严格的Th2样细胞因子反应。
