人疱疹病毒6型糖蛋白H上一个变异的B特异性中和表位的鉴定

Identification of a variant B-specific neutralizing epitope on glycoprotein H of human herpesvirus-6.

作者信息

Takeda K, Haque M, Sunagawa T, Okuno T, Isegawa Y, Yamanishi K

机构信息

Department of Microbiology, Osaka University Medical School, Osaka University, Japan.

出版信息

J Gen Virol. 1997 Sep;78 ( Pt 9):2171-8. doi: 10.1099/0022-1317-78-9-2171.

DOI:10.1099/0022-1317-78-9-2171

PMID:9292004

Abstract

We have identified the human herpesvirus-6 variant B (HHV-6B)-specific neutralizing epitope on glycoprotein H (gH) which is recognized by monoclonal antibody (MAb) OHV3, with complement-independent neutralizing activity. HHV-6 gHs from HHV-6A (strain U1102) and HHV-6B (strain HST) were expressed in a T7-vaccinia virus transient expression system. OHV3 reacted with HST gH, but not with U1102 gH, in an immunoprecipitation assay and an indirect immunofluorescence assay. In addition, OHV3 reacted with chimeric gHs, formed between U1102 gH and HST gH, containing amino acids 272 to 422 of HST gH. Sequence comparison between U1102 and HST showed seven amino acid differences in this region. Site-specific mutations were introduced into these positions and then reactivity against OHV3 was investigated. The arginine at position 389 of HST gH was shown to be a determinant of the HHV-6B-specific reactivity of OHV3.

摘要

我们已经鉴定出糖蛋白H（gH）上的人疱疹病毒6型B（HHV-6B）特异性中和表位，该表位可被具有补体非依赖性中和活性的单克隆抗体（MAb）OHV3识别。来自HHV-6A（U1102株）和HHV-6B（HST株）的HHV-6 gH在T7-痘苗病毒瞬时表达系统中表达。在免疫沉淀试验和间接免疫荧光试验中，OHV3与HST gH反应，但不与U1102 gH反应。此外，OHV3与在U1102 gH和HST gH之间形成的嵌合gH反应，该嵌合gH包含HST gH的第272至422位氨基酸。U1102和HST之间的序列比较显示该区域有七个氨基酸差异。在这些位置引入位点特异性突变，然后研究其对OHV3的反应性。结果表明，HST gH第389位的精氨酸是OHV3对HHV-6B特异性反应性的决定因素。