Saito T, Zhang Z J, Shibamori Y, Ohtsubo T, Noda I, Yamamoto T, Saito H
Department of Otolaryngology, Fukui Medical School, Japan.
Neurosci Lett. 1997 Aug 22;232(1):41-4. doi: 10.1016/s0304-3940(97)00574-0.
Expression of P-glycoprotein (P-gp) was detected by immunohistochemical staining and Western blot analysis in the peripheral nerves (7th and 8th nerves) of the guinea pig using anti-P-gp monoclonal antibody C219. P-gp was detected in the capillary endothelial cells of these nerves. Immunoreactivity in these nerves was similar to that in the brain. Besides these nerves, positive staining was observed in the sciatic nerve, although immunoreactivity was somewhat lower than that of the 7th and 8th nerves. The present investigation suggested that P-gp in the endothelial cells of the peripheral nerves might play a very important role as a part of the blood-nerve barrier function, since P-ag acts as an extension pump in these cells.
使用抗P-糖蛋白单克隆抗体C219，通过免疫组织化学染色和蛋白质免疫印迹分析，检测豚鼠外周神经（第7和第8神经）中P-糖蛋白（P-gp）的表达。在这些神经的毛细血管内皮细胞中检测到P-gp。这些神经中的免疫反应性与大脑中的相似。除了这些神经外，在坐骨神经中也观察到阳性染色，尽管免疫反应性略低于第7和第8神经。本研究表明，外周神经内皮细胞中的P-gp可能作为血神经屏障功能的一部分发挥非常重要的作用，因为P-ag在这些细胞中充当外排泵。
