Cuba C A, Torno C O, Ledesma O, Visciarelli E, Garcia S, Prat M I, Costamagna R, Barbieri L, Evans D A
Universidade de Brasilia, D.F. Brazil.
Rev Inst Med Trop Sao Paulo. 1996 Nov-Dec;38(6):413-21. doi: 10.1590/s0036-46651996000600005.
Diagnostic and parasite characterization and identification studies were carried out in human patients with cutaneous leishmaniasis lesions in Santiago del Estero, Northern Province of Argentina. Diagnostic procedures were biopsies of lesions for smears and inoculations in hamster, needle aspirations of material from ulcers for "in vitro" cultures. Immunodiagnostic techniques applied were IFAT-IgG and Montenegro skin test. Primary isolation of eight stocks of leishmanial parasites was achieved from patients with active lesions. All stocks were biologically characterized by their behaviour in hamster, measurements of amastigote and promastigotes and growth "in vitro". Eight stocks were characterized and identified at species level by their reactivity to a cross-panel of sub-genus and species-specific Monoclonal Antibodies through an Indirect Immunofluorescence technique and a Dot-ELISA. We conclude from the serodeme analysis of Argentina stocks that: stocks MHOM/AR/92/SE-1; SE-2; SE-4; SE-8; SE-8-I; SE-30; SE-34 and SE-36 are Leishmania (Viannia) braziliensis. Three Leishmania stocks (SE-1; SE-2 and SE-30) did not react with one highly species-specific Monoclonal Antibody (Clone: B-18, Leishmania-(Viannia) braziliensis marker) disclosing two serodeme group patterns. Five out of eight soluble extracts of leishmanial promastigotes were electrophoresed on thin-layer starch gels and examined for the enzyme MPI, Mannose Phosphate Isomerase; MDH, Malate Dehydrogenase; 6PGD, 6 Phosphogluconate Dehydrogenase; NH, Nucleoside Hydrolase, 2-deoxyinosine as substrate; SOD, Superoxide Dismutase; GPI, Glucose Phosphate Isomerase and ES, Esterase. From the isoenzyme studies we concluded that stocks: MHOM/AR/92/SE-1; SE-2; SE-4; SE-8 and SE-8-I are isoenzymatically Leishmania (Viannia) braziliensis. We need to analyze more enzymes before assigning them to a braziliensis zymodeme.
在阿根廷北部省份圣地亚哥 - 德尔埃斯特罗患有皮肤利什曼病损伤的人类患者中开展了诊断以及寄生虫特征和鉴定研究。诊断程序包括对病变进行活检以制作涂片并接种到仓鼠体内，对溃疡处的物质进行针吸以用于“体外”培养。应用的免疫诊断技术为间接荧光抗体试验 - IgG和蒙氏皮肤试验。从患有活动性病变的患者中首次分离出8株利什曼原虫寄生虫株。所有虫株都通过其在仓鼠体内的行为、无鞭毛体和前鞭毛体的测量以及“体外”生长情况进行生物学特性鉴定。通过间接免疫荧光技术和斑点酶联免疫吸附测定，利用一组亚属和种特异性单克隆抗体的反应性，对8株虫株在种水平上进行了特征鉴定和识别。我们从对阿根廷虫株的血清型分析得出以下结论：虫株MHOM/AR/92/SE - 1、SE - 2、SE - 4、SE - 8、SE - 8 - I、SE - 30、SE - 34和SE - 36为巴西利什曼原虫（维氏亚属）。三株利什曼原虫虫株（SE - 1、SE - 2和SE - 30）不与一种高度种特异性单克隆抗体（克隆：B - 18，巴西利什曼原虫（维氏亚属）标志物）发生反应，揭示了两种血清型组模式。对8株利什曼原虫前鞭毛体的可溶性提取物中的5种进行了薄层淀粉凝胶电泳，并检测了磷酸甘露糖异构酶（MPI）、苹果酸脱氢酶（MDH）、6 - 磷酸葡萄糖酸脱氢酶（6PGD）、核苷水解酶（以2 - 脱氧肌苷为底物）（NH）、超氧化物歧化酶（SOD）、葡萄糖磷酸异构酶（GPI）和酯酶（ES）。从同工酶研究中我们得出结论：虫株MHOM/AR/92/SE - 1、SE - 2、SE - 4、SE - 8和SE - 8 - I在同工酶方面为巴西利什曼原虫（维氏亚属）。在将它们归入巴西利什曼原虫酶型之前，我们需要分析更多的酶。
