通过同工酶电泳对巴西利什曼原虫（维阿尼亚种）和秘鲁利什曼原虫（维阿尼亚种）进行生化特性分析。

Biochemical characterization of Leishmania (Viannia) braziliensis and Leishmania (Viannia) peruviana by isoenzyme electrophoresis.

作者信息

Arana M, Evans D A, Zolessi A, Cuentas A L, Arevalo J

机构信息

Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru.

出版信息

Trans R Soc Trop Med Hyg. 1990 Jul-Aug;84(4):526-9. doi: 10.1016/0035-9203(90)90025-a.

DOI:10.1016/0035-9203(90)90025-a

PMID:2091345

Abstract

Leishmanial organisms isolated from 24 patients with Andean cutaneous leishmaniasis (uta) and from 7 with sylvatic leishmaniasis in both cutaneous and mucosal forms were characterized on the basis of their isoenzyme profiles for 13 enzymes using both cellulose acetate (CA) and thin-layer starch gel (TLS) electrophoretic techniques. Malate dehydrogenase (MDH) after electrophoresis on CA or TLS and mannose phosphate isomerase (MPI) on TLS were the only enzymes of 13 examined which discriminated between the organisms from patients with uta (L. (V.) peruviana) and those with sylvatic leishmaniasis (L. (V.) braziliensis). Mannose phosphate isomerase gave more clear-cut and reproducible discrimination than did MDH on either TLS or CA, and it is suggested that MPI is a reliable enzyme marker that can be used in routine TLS electrophoresis to distinguish between L. (V.) peruviana and L. (V). braziliensis.

摘要

从24例患有安第斯皮肤利什曼病（uta）的患者以及7例患有皮肤和黏膜型丛林利什曼病的患者中分离出利什曼原虫生物体，利用醋酸纤维素（CA）和薄层淀粉凝胶（TLS）电泳技术，基于13种酶的同工酶谱对其进行了表征。在CA或TLS上进行电泳后的苹果酸脱氢酶（MDH）以及在TLS上的磷酸甘露糖异构酶（MPI）是所检测的13种酶中仅有的能够区分uta患者（秘鲁利什曼原虫[Viannia亚属]）和丛林利什曼病患者（巴西利什曼原虫[Viannia亚属]）体内生物体的酶。在TLS或CA上，磷酸甘露糖异构酶比MDH给出的区分更清晰且可重复，并且有人提出MPI是一种可靠的酶标记物，可用于常规TLS电泳以区分秘鲁利什曼原虫[Viannia亚属]和巴西利什曼原虫[Viannia亚属]。