钙调蛋白，一种黑腹果蝇的钠钙交换基因。
Calx, a Na-Ca exchanger gene of Drosophila melanogaster.
作者信息
Schwarz E M, Benzer S
机构信息
Division of Biology, 156-29, California Institute of Technology, Pasadena, CA 91125, USA.
出版信息
Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10249-54. doi: 10.1073/pnas.94.19.10249.
Abstract
We have cloned Calx, a gene that encodes a Na-Ca exchanger of Drosophila melanogaster. Calx encodes two repeated motifs, Calx-alpha and Calx-beta, that overlap domains required for exchanger activity and regulation. Calx has multiple transcripts in adults, including at least one expressed in the retina. The Calx genomic locus comprises >/=35 kb between the Atpalpha and rudimentary-like genes in chromosomal region 93B. In Xenopus oocytes, microinjected Calx cRNA induces calcium uptake like that of its homolog, the 3Na+-1Ca2+ exchanger of mammalian heart. Implications of Calx-alpha motifs for the mechanism of Na-Ca exchange are discussed.
摘要
我们克隆了Calx基因，该基因编码果蝇的一种钠钙交换蛋白。Calx编码两个重复基序，即Calx-α和Calx-β，它们与交换蛋白活性和调节所需的结构域重叠。Calx在成虫中有多种转录本，包括至少一种在视网膜中表达的转录本。Calx基因座在染色体区域93B的Atpalpha基因和类残基基因之间包含≥35 kb。在非洲爪蟾卵母细胞中，显微注射的Calx cRNA诱导钙摄取，类似于其同源物——哺乳动物心脏的3Na+-1Ca2+交换蛋白。文中讨论了Calx-α基序对钠钙交换机制的影响。
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2
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