Goyal H O, Bartol F F, Wiley A A, Khalil M K, Chiu J, Vig M M
Department of Biomedical Sciences, Tuskegee University, AL 36088, USA.
Anat Rec. 1997 Sep;249(1):54-62. doi: 10.1002/(SICI)1097-0185(199709)249:1<54::AID-AR7>3.0.CO;2-F.
Because of the significance of androgens and estrogens in prenatal and postanatal differentiation of the testis and excurrent ducts, it is important to understand the developmental pattern of androgen receptor (AR) and estrogen receptor (ER) in these organs.
Tissues from 1-23-week-old goats were fixed in 4% paraformaldehyde and embedded in Paraplast-plus. Antigenic sites for AR and ER were immunolocalized using the PG-21 rabbit anti-rat/human antibody and the H-222 rat anti-human monoclonal antibody, respectively. The avidin-biotin horseradish peroxidase procedure was used to identify positive immunoreactivity. Controls included incubation of sections with irrelevant IgG in place of primary antibody.
Within the testis, immunostaining for AR in the nuclei of Sertoli cells increased gradually from mild at week 1 to strong at week > or = 19. In contrast, nuclei of peritubular myoid cells and Leydig cells exhibited moderate to strong reaction for AR in all animals. Germ cells were negative. Within the rete testis, efferent ductules, regions I-V of the epididymis, and ductus deferens, nuclei of all epithelial cells, peritubular myoid cells, and intertubular connective tissue cells expressed moderate to strong staining for AR at all ages. ER were confined to nonciliated cells of the efferent ductules, which displayed moderate staining in all animals, beginning from week 1.
Nuclear AR staining, found in all testicular cells (except germ cells) and excurrent duct cells examined, was observed to change in an age-related manner only in Sertoli cells, where staining intensity increased between week 1 and week 19. Staining for ER, confined to nonciliated epithelial cells of the efferent ductules, was not affected by postnatal age.
由于雄激素和雌激素在睾丸及输出管道的产前和产后分化中具有重要意义，了解这些器官中雄激素受体（AR）和雌激素受体（ER）的发育模式很重要。
将1至23周龄山羊的组织固定于4%多聚甲醛中，并包埋于Paraplast-plus中。分别使用PG-21兔抗大鼠/人抗体和H-222大鼠抗人单克隆抗体对AR和ER的抗原位点进行免疫定位。采用抗生物素蛋白-生物素辣根过氧化物酶法鉴定阳性免疫反应。对照包括用无关IgG代替一抗孵育切片。
在睾丸内，支持细胞细胞核中AR的免疫染色从第1周的轻度逐渐增加到≥19周时的强阳性。相比之下，在所有动物中，睾丸肌样细胞和间质细胞的细胞核对AR表现出中度至强反应。生殖细胞为阴性。在睾丸网、输出小管、附睾I-V区和输精管中，所有上皮细胞、睾丸肌样细胞和管间结缔组织细胞的细胞核在所有年龄段均表达中度至强染色的AR。ER局限于输出小管的非纤毛细胞，从第1周开始，在所有动物中均显示中度染色。
在所检查的所有睾丸细胞（生殖细胞除外）和输出管道细胞中均发现核AR染色，仅在支持细胞中观察到其以与年龄相关的方式变化，其中染色强度在第1周和第19周之间增加。局限于输出小管非纤毛上皮细胞的ER染色不受出生后年龄的影响。
