Douet J P, Castroviejo M, Dodin A, Bébéar C
Direction Générale de la Concurrence de la Consommation et de la Répression des Fraudes (DGCCRF), Université de Bordeaux I, Talence, France.
Res Microbiol. 1996 Nov-Dec;147(9):687-96. doi: 10.1016/s0923-2508(97)85116-9.
The haemolytic action of 125I-labelled thermostable direct haemolysin from Vibrio parahaemolyticus was studied on human and equine erythrocytes. In the first step, the haemolysin bound to the membranes of both erythrocyte species. This binding seemed temperature-independent. Then, for human erythrocytes, haemolysin produced cell disruption, and haemoglobin was released. Following this step, haemolysin was also released in a temperature-dependent manner. In contrast, equine erythrocytes were not disrupted, and no release of haemolysin occurred. The receptors of labelled haemolysin were analysed by assaying the lipid/toxin interaction on a nylon membrane and by binding on thin-layer chromatograms. the ganglioside asialo-GM2 was found to be the most potent receptor, but asialo-GM1 and lactocerebroside may also have been involved.
研究了副溶血性弧菌125I标记的耐热直接溶血素对人和马红细胞的溶血作用。第一步，溶血素与两种红细胞的膜结合。这种结合似乎与温度无关。然后，对于人红细胞，溶血素导致细胞破裂，血红蛋白释放。此步骤之后，溶血素也以温度依赖的方式释放。相比之下，马红细胞未被破坏，也没有溶血素释放。通过在尼龙膜上测定脂质/毒素相互作用以及在薄层色谱图上进行结合分析标记溶血素的受体。发现神经节苷脂脱唾液酸GM2是最有效的受体，但脱唾液酸GM1和乳糖脑苷脂可能也参与其中。
