Kato K, Nakagaki H, Takami Y, Tsuge S, Ando S, Robinson C
Department of Preventive Dentistry and Dental Public Health, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.
Arch Oral Biol. 1997 Jul;42(7):521-5. doi: 10.1016/s0003-9969(97)00046-0.
A new sampling method, capable of sampling plaque from its surface to its interior for quantitative studies, was modified to meet some of the requirements for the determination of the fluoride and mineral (Ca and P) profiles within dental plaque formed in vivo. Plaque samples were repeatedly collected from the same individual, using special devices, before a single fluoride rinse (900 parts/10(6) fluoride) and 10 min and 24 hr after rinse. The method allowed examination of fluoride, calcium and phosphorus distribution along the entire thickness of plaque. Fluoride content significantly increased throughout the sample 10 min after rinsing, indicating the fluoride had rapidly penetrated into the plaque. Although the elevated fluoride concentrations diminished almost to baseline with 24 hr, a high correlation was found between fluoride and minerals in each plaque fraction. It is concluded that this technique will be useful for evaluating the fluoride and mineral behaviour in the saliva/plaque and plaque/enamel interfaces, and the anti caries efficacy of fluoride applications.
一种能够从菌斑表面到内部进行采样以进行定量研究的新采样方法，经过改进以满足一些体内形成的牙菌斑中氟化物和矿物质（钙和磷）分布测定的要求。使用特殊装置，在单次氟化物冲洗（900 ppm氟化物）前以及冲洗后10分钟和24小时，从同一个体反复采集菌斑样本。该方法允许检查氟化物、钙和磷在菌斑整个厚度上的分布。冲洗后10分钟，整个样本中的氟化物含量显著增加，表明氟化物已迅速渗透到菌斑中。尽管24小时后升高的氟化物浓度几乎降至基线，但在每个菌斑部分中氟化物与矿物质之间发现高度相关性。结论是，该技术将有助于评估唾液/菌斑和菌斑/牙釉质界面中氟化物和矿物质的行为，以及氟化物应用的防龋效果。
