Milenković P, Ruvidić R, Pavlović-Kentera V
Strahlentherapie. 1977 Nov;153(11):780-5.
The cell cycle time of erythroid cells was determined in normal mice and in polycythaemic mice after stimulation of erythropoiesis by exogenous erythropoietin. The method employed was 3HTdR autoradiography of bone marrow cells. After 21 or 38 hours of administratering the erythropoietin 3HTdR was injected into the animals. The cell cycle time was calculated from the curves of changes in the labelling indices of proerythroblasts and basophilic erythroblasts at one hour intervals. The cell cycle time in normal animals was 8.5 hours. Twenty-two to twenty-five hours after stimulation with 1.5 units of erythropoietin, the cell cycle was 7.8 hours. Thirty-nine to forty-two hours after injection, at the time when the number of nucleated erythroid cells was within normal limits, the cell cycle time was 7.7 hours. Under the used experimental conditions, the cell cycle time of early erythroid cells was not significantly changed in comparison with the values obtained in non-treated animals. This is in contrast to the results reported when extreme stimulation of erythropoiesis with endogenous erythropoietin was used.
在正常小鼠以及通过外源性促红细胞生成素刺激红细胞生成后的多血质小鼠中，测定了红系细胞的细胞周期时间。所采用的方法是对骨髓细胞进行³H-TdR放射自显影。在给予促红细胞生成素21或38小时后，向动物体内注射³H-TdR。根据早幼红细胞和嗜碱性红细胞标记指数每隔一小时的变化曲线计算细胞周期时间。正常动物的细胞周期时间为8.5小时。用1.5单位促红细胞生成素刺激22至25小时后，细胞周期为7.8小时。注射后39至42小时，当有核红细胞数量在正常范围内时，细胞周期时间为7.7小时。在所采用的实验条件下，与未处理动物获得的值相比，早期红系细胞的细胞周期时间没有显著变化。这与使用内源性促红细胞生成素极度刺激红细胞生成时所报道的结果形成对比。
