Beyer T, Siim J Ch, Hutchison W M
Tsitologiia. 1977 Jul;19(7):809-12.
Three methods were used to detect acid phosphatase in toxoplasma endodozoites of strain SS-119 harvested on day 4 after mouse inoculation. The modified lead nitrate method detected the enzymatic activity in host cells only; Gomori metal salt technique revealed the enzyme almost exclusively in the parasite, whereas the Standard Naphthol AS-BI phosphate method suggested the presence of the enzyme simultaneously in the two, though not regularly in the parasites. The enzymatic activity when present was visualized as few coloured granules mainly in the anterior cytoplasmic area on the body and at the periphery, leaving the nuclear zone unstained. Alkaline phosphatase distribution, detected with one method only (Standard Naphtol AS-BI phosphate), appeared less convincing in the parasite than in the host cell. In host cells harbouring endozoites, no increased activity was observed in the immediate closeness of the intracellular parasites.
采用三种方法检测小鼠接种后第4天收获的SS - 119株弓形虫内殖子中的酸性磷酸酶。改良硝酸铅法仅在宿主细胞中检测到酶活性；Gomori金属盐技术显示该酶几乎仅存在于寄生虫中，而标准萘酚AS - BI磷酸酯法表明该酶同时存在于两者中，不过在寄生虫中并不规律。当存在酶活性时，可见为少数主要位于虫体前部细胞质区域和周边的有色颗粒，核区不着色。仅用一种方法（标准萘酚AS - BI磷酸酯法）检测的碱性磷酸酶分布，在寄生虫中的表现不如在宿主细胞中令人信服。在含有内殖子的宿主细胞中，在细胞内寄生虫紧邻区域未观察到活性增加。
