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[刚地弓形虫生命周期不同阶段的细胞化学研究。VII. 包囊形式中的氧化还原酶]

[Cytochemical study of different stages in the life cycle of Toxoplasma gondii. VII. Oxidation--reduction enzymes in the cyst forms].

作者信息

Beyer T V, Siim J C, Hutchison U M

出版信息

Tsitologiia. 1977 Nov;19(11):1261-5.

PMID:203066
Abstract

Dehydrogenases of glycolysis, Kreb's cycle, and pentose-phosphate shunt were detected in cystozoites of Toxoplasma gondii strain SS-119 with various degrees of activity. A mixed oxidative metabolism may be postulated on this stage of the toxoplasma life cycle. Besides, the activity of cytochrome oxidase was detected in cystozoites; the addition of cytochrome c to the incubation medium significantly intensified the reaction intensity. Of interest seems the observation of a layer of higher enzymatic activity in the host brain tissue in the immediate neighbourhood with the cyst body. This may be regarded as the host cells' (or tissue') response to the presence of the parasite's alien body.

摘要

在刚地弓形虫SS - 119株的包囊型虫体中检测到糖酵解、三羧酸循环和磷酸戊糖途径的脱氢酶,其活性程度各异。据此推测在弓形虫生命周期的这一阶段可能存在混合氧化代谢。此外,在包囊型虫体中检测到细胞色素氧化酶的活性;向孵育培养基中添加细胞色素c可显著增强反应强度。值得关注的是,在紧邻包囊体的宿主脑组织中观察到一层酶活性较高的区域。这可被视为宿主细胞(或组织)对寄生虫异物存在的反应。

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Enzymatic activities of Toxoplasma gondii.刚地弓形虫的酶活性
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