Polishchuk S M, Gura S M, Meged' O F
Ukr Biokhim Zh. 1977 Nov-Dec;49(6):28-33.
Blood serum of oncologic patients due to immunoglobulin involved in its composition, activates glycolysis in the soluble fraction of muscles when using starch, glycogen and glucose as substrates. The activation is registered under both aerobic and anaerobic conditions. When elucidating the immunoglobulin effect in a glycolytic chain under aerobic conditions it is shown that its activating effect in the incomplete incubation system is manifested with such glycolysis substrates as fructose-6-phosphate and 2-phosphoglyceric acid. Glycolysis activation with serum is insignificant or absent at all with the presence of glucose-6-phosphate, fructose-1,6-diphosphate, 3-phosphoglyceric aldehide, 3-phosphoglyceric acid, phosphoenolpyruvic acid, sodium pyruvate. Immunoglobulin isolated from the blood serum of oncologic patients does not affect the activity of purified preparations of hexokinase, glycerinaldehydephosphate dehydrogenase, lactate dehydrogenase under aerobic and anaerobic conditions. When using the air as a gas medium lactate dehydrogenase is activated by immunoglobulin. Lactate dehydrogenase activity under aerobic and anaerobic conditions is essentially lower than in the case when the air serves as a gas medium.
由于肿瘤患者血清成分中含有免疫球蛋白，当以淀粉、糖原和葡萄糖作为底物时，其能激活肌肉可溶性部分中的糖酵解过程。在有氧和无氧条件下均能检测到这种激活作用。在有氧条件下阐明免疫球蛋白在糖酵解链中的作用时发现，其在不完全孵育系统中的激活作用在诸如6-磷酸果糖和2-磷酸甘油酸等糖酵解底物中表现出来。在存在6-磷酸葡萄糖、1,6-二磷酸果糖、3-磷酸甘油醛、3-磷酸甘油酸、磷酸烯醇丙酮酸、丙酮酸钠的情况下，血清对糖酵解的激活作用不明显或完全不存在。从肿瘤患者血清中分离出的免疫球蛋白在有氧和无氧条件下均不影响纯化的己糖激酶、甘油醛磷酸脱氢酶、乳酸脱氢酶制剂的活性。当以空气作为气体介质时，乳酸脱氢酶会被免疫球蛋白激活。有氧和无氧条件下乳酸脱氢酶的活性明显低于以空气作为气体介质时的情况。
