Mouvet C, Broussard S, Riolland H, Baran N, Abuknesha R, Ismail G
BRGM, Geochemistry and Physical Chemistry Department, Orléans.
Chemosphere. 1997 Sep;35(5):1099-116. doi: 10.1016/s0045-6535(97)00175-6.
Trials were carried out on the commercially available Millipore isoproturon ELISA microtiter plate tests and on laboratory assays developed by Hirst as part of a Joint European Union research project (BIOPTICAS). The lowest detectable dose (LDD) was determined as three times the standard deviation of the blanks. Depending on the calibration curves obtained on different days with different plates, the LDD varied from 0.020 to 0.064 microgram/L for the Millipore test and from 0.080 to 0.329 microgram/L for the Hirst test. The mean coefficients of variation within a single plate for triplicate determinations of standard solutions in the 0.05 to 0.5 microgram/L range were 5.5 and 3.6% for Millipore and Hirst respectively. Cross-reactivity was studied for mono- and di-demethylated isoproturon, chlortoluron, diuron and linuron. The highest cross-reactivity with both tests was that of mono-demethylated isoproturon (22% for Millipore, 4% for Hirst). This molecule was the only one to show significant cross-reactivity in the Hirst test, whereas in the Millipore test, the di-demethylated isoproturon also cross-reacted (4%). Natural water samples, 19 ground-, 53 lysimetric plate and 47 suction cup water samples, and 32 soil samples were also analysed with the ELISA tests. HPLC with a diode array detector was used as a validated control technique for the natural samples. For each water type, ELISA concentrations of both tests were significantly correlated with the HPLC values (r > or = 0.937; p < 0.001). For the soil extracts, the correlations were also significant (p < 0.001), but the scatter in the data was greater. Overall, the Millipore correlation coefficients were higher than those of Hirst.
针对市售的密理博异丙隆酶联免疫吸附测定（ELISA）微量滴定板检测以及赫斯特开发的实验室检测方法进行了试验，这些检测方法是欧盟联合研究项目（BIOPTICAS）的一部分。最低检测剂量（LDD）确定为空白值标准差的三倍。根据在不同日期使用不同平板获得的校准曲线，密理博检测的LDD在0.020至0.064微克/升之间变化，赫斯特检测的LDD在0.080至0.329微克/升之间变化。对于0.05至0.5微克/升范围内标准溶液的一式三份测定，密理博和赫斯特在单个平板内的平均变异系数分别为5.5%和 3.6%。研究了单去甲基化和双去甲基化异丙隆、绿麦隆、敌草隆和利谷隆的交叉反应性。两种检测方法中交叉反应性最高的是单去甲基化异丙隆（密理博为22%，赫斯特为4%）。该分子是赫斯特检测中唯一显示出显著交叉反应性的物质，而在密理博检测中，双去甲基化异丙隆也有交叉反应（4%）。还使用ELISA检测分析了天然水样（19份地下水样、53份测渗仪板水样和47份吸盘水样）以及32份土壤样品。配备二极管阵列检测器的高效液相色谱法（HPLC）用作天然样品的验证对照技术。对于每种水样类型，两种检测方法的ELISA浓度与HPLC值均显著相关（r≥0.937；p<0.001）。对于土壤提取物，相关性也很显著（p<0.001），但数据的离散度更大。总体而言，密理博的相关系数高于赫斯特的相关系数。
