Shearman L P, Zeitzer J, Weaver D R
Laboratory of Developmental Chronobiology, Massachusetts General Hospital, Jackson, Boston 02114, USA.
Brain Res Dev Brain Res. 1997 Aug 18;102(1):105-15. doi: 10.1016/s0165-3806(97)00091-6.
Maternal treatment with cocaine or the D1-dopamine receptor agonist, SKF 38393, induces expression of the immediate-early gene, c-fos, in fetal rodent brain. Our previous studies have focused on the suprachiasmatic nucleus late in gestation. In the present report, we examined the anatomical distribution of functional D1-dopamine receptors throughout fetal rat brain. Functional D1 receptors were defined using three complementary methods: in situ hybridization to detect D1 receptor mRNA, autoradiographic detection of 125I-SCH 23982 binding, and in situ hybridization to detect c-fos gene expression induced by maternal treatment with SKF 38393. D1-dopamine receptor binding, receptor mRNA, and SKF 38393-induced c-fos gene expression are widespread in fetal brain by late gestation. These data indicate that the fetal brain is sensitive to dopamine receptor activation, and suggest that gestational exposure to drugs of abuse acting via dopaminergic mechanisms may influence fetal brain function.
用可卡因或D1-多巴胺受体激动剂SKF 38393对母体进行治疗，会诱导胎鼠大脑中即早基因c-fos的表达。我们之前的研究集中在妊娠后期的视交叉上核。在本报告中，我们研究了功能性D1-多巴胺受体在整个胎鼠大脑中的解剖分布。功能性D1受体通过三种互补方法进行定义：原位杂交检测D1受体mRNA、放射自显影检测125I-SCH 23982结合，以及原位杂交检测母体用SKF 38393治疗诱导的c-fos基因表达。到妊娠后期，D1-多巴胺受体结合、受体mRNA和SKF 38393诱导的c-fos基因表达在胎脑中广泛存在。这些数据表明，胎脑对多巴胺受体激活敏感，并提示孕期接触通过多巴胺能机制起作用的滥用药物可能会影响胎脑功能。
