Viazov S, Kuzin S, Paladi N, Tchernovetsky M, Isaeva E, Mazhul L, Vasychova F, Widell A, Roggendorf M
Ivanovsky Institute of Virology, Moscow, Russia.
J Med Virol. 1997 Sep;53(1):36-40.
The prevalence of HCV genotypes in four republics of the former Soviet Union (Russia, Belarus, Moldova, and Uzbekistan) was investigated. Overall, 197 HCV isolates from 66 blood donors and 131 patients with chronic hepatitis were typed. Viral sequences from sera of infected subjects were amplified by nested RT-PCR using primers from the core region and typed by one or two techniques: (1) DNA enzyme immunoassay (DEIA) and (2) PCR with a set of type-specific primers. Only three major HCV genotypes were identified in this study population. HCV 1b was found to be the predominant virus type both among blood donors and chronic hepatitis patients, followed by 3a, 2a, and 1a (chronic hepatitis patients: 1b-82%; 3a-10%; 2a-4%, 1a-5% and 2c-1%; blood donors: 1b-77%; 3a-17%; and 2a-6%). No significant difference in genotype distribution was observed between different countries or between blood donors and chronic hepatitis patients within the same country. Results of the genotyping procedures were confirmed by direct sequencing of 216 nt PCR fragments corresponding to part of HCV core gene. Phylogenetic analysis of HCV 1b sequences from this study and from the Genbank demonstrated that the sequences from the former Soviet Union do not form evolutionary lineage(s) different from those of strains of the same subtype circulating in other geographical regions.
对前苏联四个共和国（俄罗斯、白俄罗斯、摩尔多瓦和乌兹别克斯坦）丙型肝炎病毒（HCV）基因型的流行情况进行了调查。总共对来自66名献血者和131名慢性肝炎患者的197株HCV分离株进行了分型。使用来自核心区域的引物，通过巢式逆转录聚合酶链反应（RT-PCR）扩增受感染受试者血清中的病毒序列，并通过一种或两种技术进行分型：（1）DNA酶免疫测定（DEIA）和（2）使用一组型特异性引物的PCR。在该研究人群中仅鉴定出三种主要的HCV基因型。发现HCV 1b是献血者和慢性肝炎患者中主要的病毒类型，其次是3a、2a和1a（慢性肝炎患者：1b-82%；3a-10%；2a-4%，1a-5%，2c-1%；献血者：1b-77%；3a-17%；2a-6%）。在不同国家之间或同一国家内的献血者和慢性肝炎患者之间，未观察到基因型分布的显著差异。通过对与HCV核心基因部分相对应的216 nt PCR片段进行直接测序，证实了基因分型程序的结果。对本研究和Genbank中HCV 1b序列的系统发育分析表明，来自前苏联的序列与在其他地理区域流行的相同亚型毒株的序列没有形成不同的进化谱系。
