Naito S, Hoshi M, Mashimo S
Biological Science Laboratories, Kao Corporation, 2606 Akabane, Ichikai, Haga, Tochigi, 321-34, Japan.
Anal Biochem. 1997 Sep 5;251(2):163-72. doi: 10.1006/abio.1997.2256.
A method of in vivo analysis of the free water content in living organisms by dielectric analysis in the time domain is described. Human skin is chosen as an example of living tissue. The cells suitable for the measurement of various layers of human skin and calculation procedures for the waveform reflected from the probe end are described. The approach was confirmed to be effective for the determination of the water content through measurement of the standard samples, keratin-water mixtures. This method was also applied to human skin in vivo. Water content data measured with a probe specially designed for surface layer analysis were sensitive to humidity around the subject. The formula expressing the relation between the electrical field character of the probe, the permittivity depth profile, and the measured permittivity was used to analyze the water content profile as a function of the depth from the skin surface. The use of several kinds of probes, differing in their electric field characteristics, permitted evaluation of the water content depth profile of human skin. This procedure is easy and applicable to any sample due to its simplicity. The measurement needs only a touch of the probe on a sample spot. It is therefore a promising method of physicochemical research on living organisms and biomaterials.
描述了一种通过时域介电分析对活生物体中的自由水含量进行体内分析的方法。以人体皮肤作为活组织的示例。描述了适用于测量人体皮肤各层的细胞以及从探头末端反射的波形的计算程序。通过测量标准样品角蛋白 - 水混合物，证实该方法对于测定水含量是有效的。该方法也被应用于人体皮肤的体内分析。用专门设计用于表层分析的探头测量的水含量数据对受试者周围的湿度敏感。用表示探头电场特性、介电常数深度分布和测量介电常数之间关系的公式来分析作为距皮肤表面深度函数的水含量分布。使用几种电场特性不同的探头，可以评估人体皮肤的水含量深度分布。由于该过程简单，所以它易于操作且适用于任何样品。测量仅需将探头轻触样品点。因此，它是一种对活生物体和生物材料进行物理化学研究的有前景的方法。
