Taq聚合酶介导的不依赖引物/模板的聚d(A-T)合成。
Primer/template-independent synthesis of poly d(A-T) by Taq polymerase.
作者信息
Hanaki K, Odawara T, Muramatsu T, Kuchino Y, Masuda M, Yamamoto K, Nozaki C, Mizuno K, Yoshikura H
机构信息
Department of Bacteriology, Faculty of Medicine, University of Tokyo, Japan.
出版信息
Biochem Biophys Res Commun. 1997 Sep 8;238(1):113-8. doi: 10.1006/bbrc.1997.7197.
Taq DNA polymerase polymerized dATP and dTTP to poly d(A-T) without requiring added primer/template in the temperature range of 60-70 degrees C. Tth DNA polymerase also catalyzed the reaction, while delta Tth, Vent, Vent(exo-), Pfu, Ultma, BcaBEST, and KOD DNA polymerases did not. The reaction was distinct from the template-nonrequiring terminal deoxynucleotidyl transferase reaction which absolutely required primers.
Taq DNA聚合酶在60 - 70摄氏度的温度范围内无需添加引物/模板就能将dATP和dTTP聚合成聚d(A - T)。Tth DNA聚合酶也能催化该反应，而delta Tth、Vent、Vent(exo-)、Pfu、Ultma、BcaBEST和KOD DNA聚合酶则不能。该反应与绝对需要引物的无需模板的末端脱氧核苷酸转移酶反应不同。
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