Ignatov K B, Kramarov V M, Uznadze O L, Miroshnikov A I
Bioorg Khim. 1997 Oct;23(10):817-22.
The ability of three thermostable enzymes, Tth, Taq, and Klentaq DNA polymerases, to amplify DNA with primers containing mismatches in the 3'-terminal region was studied. It is shown that Tth polymerase, in contrast to the Taq and Klentaq enzymes, synthesizes equally well DNA with primers perfectly complementary to the template and with those containing mismatches next the 3'-end. The use of Tth DNA polymerase in the polymerase chain reaction was shown to result, in some cases, in a great number of additional, nonspecific DNA fragments as compared with Taq DNA polymerase. This may be due to the ability of Tth polymerase for DNA primer extension even if the 3'-terminal region of the primer contains nucleotides non-complementary to the template. Tth DNA polymerase and a Klentaq/Tth mixture (100:1) can be efficiently used in the amplification of DNA with degenerated primers and primers forming nonperfect duplexes with the template.
研究了三种热稳定酶，即Tth、Taq和Klentaq DNA聚合酶，利用3'-末端区域含有错配的引物扩增DNA的能力。结果表明，与Taq和Klentaq酶不同，Tth聚合酶使用与模板完全互补的引物以及3'-末端附近含有错配的引物合成DNA的效果同样良好。与Taq DNA聚合酶相比，在聚合酶链反应中使用Tth DNA聚合酶有时会产生大量额外的非特异性DNA片段。这可能是由于即使引物的3'-末端区域含有与模板不互补的核苷酸，Tth聚合酶仍具有DNA引物延伸的能力。Tth DNA聚合酶和Klentaq/Tth混合物（100:1）可有效地用于使用简并引物和与模板形成非完美双链体的引物进行DNA扩增。
