Cuocolo R, de Martino G, Ortolani O, Conti A
Boll Soc Ital Biol Sper. 1979 Jul 30;55(14):1438-44.
An improved technique of two dimensional immunoelectrophoresis of serum proteins on gelatinized cellulose acetate for routine evaluation of protein nutritional state is described. The method, which allows the estimation of more than 30 protein fractions, proves extremely useful in monitoring the effectiveness of total parenteral nutrition and protein metabolic conditions. Sera samples from healthy subjects and cachectical neoplastic patients have been examined and preliminary results are reported.
本文描述了一种改进的血清蛋白二维免疫电泳技术,该技术在糊化醋酸纤维素上进行,用于蛋白质营养状态的常规评估。该方法能够估算30多种蛋白质组分,在监测全胃肠外营养的效果和蛋白质代谢状况方面被证明极为有用。已对健康受试者和恶病质肿瘤患者的血清样本进行了检测,并报告了初步结果。