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猪弓形虫病改良凝集试验特异性的验证

Validation of the specificity of the modified agglutination test for toxoplasmosis in pigs.

作者信息

Dubey J P

机构信息

Parasite Biology and Epidemiology Laboratory, US Department of Agriculture, Beltsville, MD 20705-2350, USA.

出版信息

Vet Parasitol. 1997 Aug;71(4):307-10. doi: 10.1016/s0304-4017(97)00016-2.

DOI:10.1016/s0304-4017(97)00016-2
PMID:9299699
Abstract

Serum samples from Toxoplasma gondii-negative pigs infected with protozoa, helminths and viruses, and from fetal pigs were examined for cross reacting antibodies to T. gondii by the use of the modified agglutination test (MAT). Nonspecific reactions with T. gondii antigen were not found in 1:10 dilutions of sera from eight pigs experimentally infected with Sarcocystis miescheriana, 11 pigs infected with Ascaris suum, three pigs infected with Trichuris suis, ten pigs infected with Trichinella spiralis, nine pigs infected with various porcine viruses, six gnotobiotic pigs, and 82 fetal pigs. Antibodies were also not found in 1:25 dilution of sera from 200 naturally aborted fetal pigs. Thus, evidence for cross reactivity to T. gondii with unrelated or related organisms using the MAT was not found.

摘要

使用改良凝集试验(MAT),检测感染原生动物、蠕虫和病毒的弓形虫阴性猪以及胎猪的血清样本中针对弓形虫的交叉反应抗体。在8只经实验感染米氏肉孢子虫的猪、11只感染猪蛔虫的猪、3只感染猪鞭虫的猪、10只感染旋毛虫的猪、9只感染各种猪病毒的猪、6只无菌猪以及82只胎猪的血清1:10稀释液中,未发现与弓形虫抗原的非特异性反应。在200只自然流产胎猪的血清1:25稀释液中也未发现抗体。因此,未发现使用MAT检测时,与无关或相关生物体存在针对弓形虫的交叉反应的证据。

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