Poelchen W, Nieber K, Illes P
Institut für Pharmakologie und Toxikologie der Universität, Leipzig, Germany.
Eur J Pharmacol. 1997 Aug 13;332(3):267-71. doi: 10.1016/s0014-2999(97)01113-8.
Intracellular recordings were made in a pontine slice preparation of the rat brain containing the nucleus locus coeruleus. The pressure application of N-methyl-D-aspartate (NMDA) produced reproducible depolarizations of stable amplitude. Superfusion with ethanol (100 mM) for 15 min inhibited the depolarizing response to NMDA: the effect of ethanol was rapidly reversed on washout. When the superfusion time of ethanol (100 mM) was increased to 60 min, its inhibitory effect disappeared after 50 to 60 min. Moreover, after the subsequent washout of ethanol a withdrawal-like increase in the sensitivity to NMDA became evident. Hence, adaptive mechanisms of locus coeruleus neurons during the long-time contact with ethanol may be modelled in an in vitro system.
在含有蓝斑核的大鼠脑桥切片标本中进行细胞内记录。施加N-甲基-D-天冬氨酸（NMDA）可产生幅度稳定的可重复去极化。用乙醇（100 mM）灌流15分钟可抑制对NMDA的去极化反应：乙醇的作用在洗脱后迅速逆转。当乙醇（100 mM）的灌流时间增加到60分钟时，其抑制作用在50至60分钟后消失。此外，在随后洗脱乙醇后，对NMDA的敏感性出现类似戒断的增加。因此，在体外系统中可以模拟蓝斑核神经元在与乙醇长期接触过程中的适应性机制。
