Sadzot-Delvaux C, Kinchington P R, Debrus S, Rentier B, Arvin A M
Department of Pediatrics, Stanford University School of Medicine, CA 94305, USA.
J Immunol. 1997 Sep 15;159(6):2802-6.
Varicella-zoster virus (VZV) is a human alpha herpesvirus that establishes latency in sensory ganglia. Latency is characterized by the abundant expression of the immediate early protein 63 (IE63), whereas other viral proteins have not yet been detected during the quiescent phase of VZV infection. The IE63 protein is a component of the virion and is expressed very early in the infectious cycle. The IE63 protein is also expressed in skin during episodes of varicella and herpes zoster. We have evaluated the cell-mediated immune response against IE63 in naturally immune adults with a history of chickenpox, by T lymphoproliferation and cytotoxicity assays. Among donors who had T cell proliferation to unfractionated VZV Ags from infected cell extract, 59% had T cell recognition of purified IE63. The CTL response to IE63 was equivalent to CTL recognition of IE62, the major tegument component of VZV whose immunogenicity has been previously described. IgG Abs against IE63 were detected in serum from healthy immune adults. These results indicate that IE63 is an important target of immunity to VZV. T cell recognition of IE63 is likely to be involved in controlling VZV reactivation from latency.
水痘带状疱疹病毒（VZV）是一种人类α疱疹病毒，可在感觉神经节中建立潜伏感染。潜伏感染的特征是立即早期蛋白63（IE63）大量表达，而在VZV感染的静止期尚未检测到其他病毒蛋白。IE63蛋白是病毒粒子的一个组成部分，在感染周期的早期就会表达。在水痘和带状疱疹发作期间，皮肤中也会表达IE63蛋白。我们通过T淋巴细胞增殖和细胞毒性试验，评估了有水痘病史的自然免疫成年人针对IE63的细胞介导免疫反应。在对来自感染细胞提取物的未分级VZV抗原产生T细胞增殖的供体中，59%的人对纯化的IE63有T细胞识别。对IE63的CTL反应等同于对IE62的CTL识别，IE62是VZV的主要被膜成分，其免疫原性此前已有描述。在健康免疫成年人的血清中检测到了针对IE63的IgG抗体。这些结果表明，IE63是VZV免疫的一个重要靶点。对IE63的T细胞识别可能参与控制VZV从潜伏状态的重新激活。
