Kulakova A N, Kulakov L A, Quinn J P
The Questor Centre and School of Biology and Biochemistry, The Queen's University of Belfast, UK.
Gene. 1997 Aug 11;195(1):49-53. doi: 10.1016/s0378-1119(97)00151-0.
The phnA gene encoding a novel carbon-phosphorus bond cleavage enzyme, phosphonoacetate hydrolase, from Pseudomonas fluorescens 23F was cloned and expressed in Escherichia coli and Pseudomonas putida. It conferred on the latter host the ability to mineralize phosphonoacetate but on the former the ability to utilize it as sole phosphorus source only. The nucleotide and deduced amino acid sequences of the phnA gene showed no significant homology with any data bank accessions.
从荧光假单胞菌23F中克隆出编码一种新型碳 - 磷键裂解酶——膦酰乙酸水解酶的phnA基因，并在大肠杆菌和恶臭假单胞菌中进行表达。该基因赋予后一种宿主矿化膦酰乙酸的能力，但赋予前一种宿主的能力仅是将其用作唯一磷源。phnA基因的核苷酸序列和推导的氨基酸序列与任何数据库收录序列均无显著同源性。
