Ferchaud V, Le Bizec B, Montrade M P, Maume D, Monteau F, André F
LDH-LNR, Ecole Nationale Vétérinaire (Ministère de l'Agriculture), Nantes, France.
J Chromatogr B Biomed Sci Appl. 1997 Aug 1;695(2):269-77. doi: 10.1016/s0378-4347(97)00124-2.
An analytical method has been developed in order to control the illegal use of stanozolol as growth promoter in livestock. The procedure was based on enzymatic hydrolysis, purification on a Clean Screen DAU column and derivatization with heptafluorobutyric anhydride prior to GC-MS analysis. This method allowed us to study the metabolism of stanozolol in cattle after oral and subcutaneous administrations. Urinary metabolites were identified by mass spectrometry. Stanozolol and 16-hydroxystanozolol were detected after oral administration, while 16-hydroxystanozolol and 4,16-dihydroxystanozolol were found after subcutaneous administration.
已开发出一种分析方法，用于控制司坦唑醇在牲畜中作为生长促进剂的非法使用。该程序基于酶水解、在Clean Screen DAU柱上纯化以及在气相色谱 - 质谱分析之前用七氟丁酸酐进行衍生化。该方法使我们能够研究司坦唑醇经口服和皮下给药后在牛体内的代谢情况。通过质谱鉴定尿液代谢物。口服给药后检测到司坦唑醇和16 - 羟基司坦唑醇，而皮下给药后发现了16 - 羟基司坦唑醇和4,16 - 二羟基司坦唑醇。
