碘乙酸和N-乙基马来酰亚胺在高效液相色谱法测定肝脏谷胱甘肽中的不同功效
Different efficacy of iodoacetic acid and N-ethylmaleimide in high-performance liquid chromatographic measurement of liver glutathione.
作者信息
Santori G, Domenicotti C, Bellocchio A, Pronzato M A, Marinari U M, Cottalasso D
机构信息
Institute of General Pathology, University of Genoa, Italy.
出版信息
J Chromatogr B Biomed Sci Appl. 1997 Aug 1;695(2):427-33. doi: 10.1016/s0378-4347(97)00159-x.
The widely used high-performance liquid chromatography (HPLC) procedure to determine glutathione in biological samples utilizing iodoacetic acid as thiol quenching agent and 1-fluoro-2,4-dinitrobenzene for derivatization has been modified regarding tissue sample processing and storage of the working solutions. The modified procedure compared with the original method reduces artifactual oxidation in rat liver glutathione measurement (1.47+/-0.8% vs. 2.84+/-0.69%, respectively). In both HPLC procedures, an increase in artifactual oxidation was found in both standard glutathione solutions and hepatic samples when N-ethylmaleimide instead of iodoacetic acid was used for thiol trapping.
利用碘乙酸作为硫醇淬灭剂、1-氟-2,4-二硝基苯进行衍生化来测定生物样品中谷胱甘肽的广泛使用的高效液相色谱（HPLC）方法，在组织样品处理和工作溶液储存方面已得到改进。与原方法相比，改进后的方法在大鼠肝脏谷胱甘肽测量中减少了人为氧化（分别为1.47±0.8%和2.84±0.69%）。在两种HPLC方法中，当使用N-乙基马来酰亚胺代替碘乙酸进行硫醇捕获时，标准谷胱甘肽溶液和肝脏样品中的人为氧化均增加。
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