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血浆或组织谷胱甘肽水平的测定方法。

Methods for the determination of plasma or tissue glutathione levels.

作者信息

Tipple Trent E, Rogers Lynette K

机构信息

Center for Perinatal Research, The Research Institute at Nationwide Children's Hospital, Columbus, OH, USA.

出版信息

Methods Mol Biol. 2012;889:315-24. doi: 10.1007/978-1-61779-867-2_20.

DOI:10.1007/978-1-61779-867-2_20
PMID:22669674
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3680121/
Abstract

We present two different methods for determining levels of glutathione in complex biological samples and plasma. The DTNB/GR enzyme recycling method is sensitive and requires no specialized equipment. The HPLC method is particularly useful for situations in which sample amounts are limited. Detailed instructions for performing each method as well as the advantages and disadvantages of each are discussed in this chapter.

摘要

我们介绍了两种用于测定复杂生物样品和血浆中谷胱甘肽水平的不同方法。5,5'-二硫代双(2-硝基苯甲酸)/谷胱甘肽还原酶（DTNB/GR）酶循环法灵敏且无需专门设备。高效液相色谱（HPLC）法在样品量有限的情况下特别有用。本章讨论了执行每种方法的详细说明以及每种方法的优缺点。

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Detection of reversible protein thiol modifications in tissues.组织中可逆蛋白质硫醇修饰的检测。
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Glutathione measurement in human plasma. Evaluation of sample collection, storage and derivatization conditions for analysis of dansyl derivatives by HPLC.人血浆中谷胱甘肽的测定。通过高效液相色谱法分析丹磺酰衍生物时样品采集、储存和衍生化条件的评估。
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